Figure 6.

cobl2 mutants display an increased solubility of the pectinaceous component of seed mucilage. A, Ten milligrams of seeds of the wild type (WT) and indicated mutants was immersed in water overnight at room temperature and occasionally shaken. B, Staining of seeds with Ruthenium Red for pectins, with gentle shaking, following pretreatment with EDTA. Note that only the adherent mucilage layer stays attached following shaking. C, Staining of seeds with Ruthenium Red with no shaking, demonstrating both adherent and nonadherent layers of seed mucilage. Bars = 2 mm (A), 0.25 mm (B), and 1 mm (C).