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. 2015 Jan 28;167(3):766–779. doi: 10.1104/pp.114.252742

Figure 1.

Figure 1.

SI-induced pollen tubes undergo rapid cytosolic acidification, and artificial alteration of pollen tube [pH]cyt can mimic and prevent SI-induced acidification. A, Pollen tubes labeled with the pH indicator BCECF AM were treated with either PrsS to provide an incompatible response (SI; n = 152) or a compatible response (compatible; n = 32) or untreated (UT; n = 69). B, Pollen tubes labeled with BCECF AM were incubated with 50 mm propionic acid (pH 7, n = 57; or pH 5.5, n = 62), pretreated with pH 7 for 10 min before SI induction (pH 7 & SI; n = 29), or untreated (n = 70).