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. 2015 Jan 28;167(3):766–779. doi: 10.1104/pp.114.252742

Figure 2.

Figure 2.

Pollen tube [pH]cyt can activate or prevent DEVDase/caspase-3-like activity. A, Representative pollen tube after SI induction labeled with FAM-DEVD-FMK FLICA exhibits fluorescence, indicating DEVDase/caspase-3-like activity. B, Bright-field image of A. C, Representative untreated pollen tube labeled with FAM-DEVD-FMK FLICA exhibits no fluorescence. D, Bright-field image of C. All images are 5 h after treatment. Bar = 10 µm. E, Quantitation of 150 pollen tubes scored positive (black bars) or negative (white bars) for fluorescence, indicating DEVDase/caspase-3-like activity, after the following treatments: SI induction (SI); growth medium (UT); 50 mm propionic acid, pH 5.5 (pH 5.5); 50 mm propionic acid, pH 7 (pH 7); or 10-min pretreatment with 50 mm propionic acid (pH 7) followed by SI induction (pH 7 & SI) incubated for 5 h before the addition of FAM-DEVD-FMK FLICA.