Fig. 4.

Effect of zebrafish nonmuscle myosin heavy chain IIA (zMyh9) knockdown on glomerular function. A: whereas Alexa fluor 647-conjugated 10-kDa dextran was almost completely cleared after 24 h from the circulation of control larvae (4 dpf), it was retained in zMyh9 knockdown larvae. The vascular signal of FITC-conjugated 500-kDa dextran remained relatively stable over time in control (Ctrl) as well as zMyh9 knockdown larvae. B: fluorescence intensity measurements over time demonstrated reduced glomerular filtration but unaffected glomerular barrier function in zMyh9 knockdown larvae. MO, morpholino oligonucleotide. Data are means ± SE of 4 larvae. Scale bar = 50 μm.