Fig. 5.

Effect of zebrafish apolipoprotein L1 (zApol1) knockdown on glomerular function. A: alexa Fluor 647-conjugated 10-kDa dextran was almost completely cleared after 24 h from the circulation of control as well as zApol1 knockdown larvae (4 dpf). The vascular signal of FITC-conjugated 500-kDa dextran remained relatively stable over time in control larvae but was practically lost in zApol1 knockdown larvae. B: fluorescence intensity measurements over time demonstrated unaffected glomerular filtration but compromised glomerular barrier function in zApol1 knockdown larvae. Data are means ± SE of 12 larvae. Scale bar = 50 μm.