A. Total RNA was extracted from renal tissues of normogycemic and hyperglycemic Tg26 mice (n=3). Subsequently, mRNA expression for angiotensinogen was determined by real time PCR studies. *P<0.001 compared to Tg26.
B. Total RNA was extracted from renal tissues of normoglycemic and hyperglycemic Tg26 mice (n=3). Subsequently, mRNA expression for renin determined by real time PCR studies. *P<0.001 compared to Tg26.
C. Renal tissues of different groups of animals (n=4) were assayed for their Ang II content by ELISA. *P<0.05 compared to FVBN; **P<0.05 compared to Tg26.