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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Cell Signal. 2014 Dec 24;27(3):460–469. doi: 10.1016/j.cellsig.2014.12.011

Figure 8. High glucose and HIV enhance podocyte DNA damage.

Figure 8

A. Empty vector- and NL4-3 (HIV)-transduced podocytes grown on cover slips were incubated in media containing either glucose (5 mM) or glucose (30 mM) for 24 hours. At the end of the incubation period, cells were co-labeled for H2AX (double strand breaks) and KU80 (DNA repair) expression and examined under a confocal microscope. Nuclei are stained blue (DAPI). Representative microphotographs are shown. Red dots in nuclei indicate double strand breaks and green dots indicate DNA repair proteins.

B. Cumulative data (number of H2AX foci) of the protocol described in A are summarized as scattergrams with lines representing the means.

C. Cumulative data (integrated density, KU80) of the protocol described in A are summarized as scattergrams with lines representing the means.