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. Author manuscript; available in PMC: 2015 Mar 3.
Published in final edited form as: Methods Mol Biol. 2014;1119:217–237. doi: 10.1007/978-1-62703-788-4_13

Table 1.

Detection of RCMV-GFP in Infected Rat Tissues*

Organ Day 3 Day 5 Day 7 Day 10 Day 21 Uninfected
Salivary Glands + + ++ ++
Heart + +/− +/− +
Lung ++ ++ + ++ +
Spleen ++ ++ ++ ++
Liver +/− ++ + +
Kidney +/− ++ + +/−
PBMC** 0.021 0.077 0.027 0 0 0
BMC 0.005 0.006 0 0 0 0
*

Groupings: no GFP+ foci in more than 5 tissue sections (−); 1–5 GFP+ foci per 5 tissue sections (+/−); multiple GFP+ foci per tissue section (+); and multiple GFP+ foci per 20× microscope field (++). n=5 rats

**

GFP positivity of PBMC and BMC was determined by flow cytometry. Listed are positive cells as a percent of total cells.