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. Author manuscript; available in PMC: 2015 Mar 3.
Published in final edited form as: J Pharm Sci Pharmacol. 2014 Sep 1;1(3):175–181. doi: 10.1166/jpsp.2014.1022

Figure 1.

Figure 1

Effect of ERG on TGF-β/Smad3 transfection activity. PNT1A cells were transiently co-transfected with p3TP reporter gene, pCMVβ and expression plasmids or parental empty expression vector as indicated. Cells were treated with or without TGF-β1 for 20 hours before harvest. Reporter activity was measured at 48 hours after the transfection. All reporter activity was shown as mean ± S.E. from three samples. The reporter activity is expressed as light units. (a), Endogenous TGF-β/smad3 activity is increased by ERG. PNT1A cells were transiently transfected with increasing amount of expression plasmid ERG. (b), The activity of TGF-β/Smad3 is increased by ERG. PNT1A cells were transiently co-transfected with expression plasmids ERG and Smad3. (c), The TGF-β/Smad3 activity is increased by increasing amount smad3 transfection. PNT1A cells were transiently transfected with increasing amount of expression plasmid Smad3. *, p<0.05; **, p < 0 01

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