Figure 2.

Endogenous phospho-Smad3 increased by ERG. (a) and (b), PNT1A cells were transfected with expression plasmid pSG5/ERG or parental empty expression vector. The cells were treated with or without TGF-β1 as shown for 2 hours (a) and for 30 minute, 1 hour, 2 hours, 4 hours 6 hours (b) before harvest. Total cell extracts were prepared and subjected to SDS-PAGE followed by western blot analysis using anti-ERG, anti-phospho-Smad3, anti-Smad3, and anti β-actin antibody.