Table 1.
Sample sheet for the assembly reagent for microRNA labeling for microarray experiments
| Amt Cy3 | Amt Cy5 | Cy3 Conc | Cy5 Conc | Cy3 | LBl | H2O | Cy5 | Lbl | H2O | Slides | Notes | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 5 | 5 | miRNA-EX_01_ | |||||||||
| 2 | 5 | 5 | miRNA-EX_01_ | |||||||||
| 3 | 5 | 5 | miRNA-EX_01_ | |||||||||
| 4 | 5 | 5 | miRNA-EX_01_ | |||||||||
| 5 | 5 | 5 | miRNA-EX_01_ | |||||||||
| 6 | 5 | 5 | miRNA-EX_01_ | |||||||||
| 7 | 5 | 5 | miRNA-EX_01_ | |||||||||
| 8 | 5 | 5 | miRNA-EX_01_ | |||||||||
| 9 | 5 | 5 | miRNA-EX_01_ | |||||||||
| 10 | 5 | 5 | miRNA-EX_01_ |
Cy3 erythrocyte RNA samples from individual patients, Cy5 common RNA reference isolated from one unit of blood, which will be used in all samples as normalization control (the dye choice for samples vs. reference is optional)