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. Author manuscript; available in PMC: 2015 Mar 4.
Published in final edited form as: Cell Signal. 2010 Apr 18;22(9):1300–1307. doi: 10.1016/j.cellsig.2010.04.006

Fig. 2. Ionizing radiation-induced mitochondrial ceramide elevations via CerS2, 5, and 6.

Fig. 2

(A) Ceramide molecular species were analyzed by mass spectrometry in mitochondria purified from control and irradiated (10 Gy) HeLa cells 33 h following exposure. Data (mean±SEM) are derived from biological duplicates, and IR-responsive ceramide species were confirmed by 2 additional independent studies. (B) RT-PCR products using CerS-specific primers on total mRNA isolated from HeLa cells were resolved on a 1% agarose gel to reveal transcripts encoding CerS1, 2, 5, and 6. These results were confirmed using a second set of homolog-specific primers as well as by real-time qPCR analyses.