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. Author manuscript; available in PMC: 2015 Mar 4.
Published in final edited form as: Cell Signal. 2010 Apr 18;22(9):1300–1307. doi: 10.1016/j.cellsig.2010.04.006

Fig. 3. CerS2 and CerS5 overexpression define opposing roles in radiation-induced mitochondrial apoptosis.

Fig. 3

(A) CerS2 overexpression partially protects HeLa cells from time-dependent IR-induced apoptosis measured by bis-benzimide staining. Data (mean±SD) are representative of 3 similar independent experiments performed in triplicate analyzing >200 cells per point. (p<0.01 vs. vector 15 Gy). Overexpression of CerS5 significantly increased apoptosis after 10 Gy, quantified in (B) by bis-benzimide staining (p<0.05 vs. vector 10 Gy) and in (C) by caspase activation (*p<0.02 vs. vector IR). Data (mean±SEM) in (B) are from 1 of 4 independent studies performed in triplicate analyzing >400 cells per point. In (C), BcL-xL overexpression abrogates both CerS5 overexpression- and IR-induced caspase activity elevations. Data (mean±SEM) in (C) are from 2 independent studies performed in duplicate. (**p<0.001 vs. vector IR). Overexpression of each CerS gene and bcl-xL was confirmed by direct western blot on cell lysate.