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. Author manuscript; available in PMC: 2015 Mar 4.
Published in final edited form as: Cell Signal. 2010 Apr 18;22(9):1300–1307. doi: 10.1016/j.cellsig.2010.04.006

Fig. 4. CerS2 confers all ionizing radiation-induced lignoceroyl-CoA-dependent CerS activity.

Fig. 4

(A) Proteins from subcellular fractions purified from HeLa cells were analyzed by SDS-PAGE/western blot for the presence of CerS2. (B) Lignoceroyl-CoA-dependent CerS activity was measured in HeLa cell homogenate prepared from cells transfected with a CerS2-specific shRNA vector 32 h after exposure to 15 Gy. Data represent 1 of 2 similar experiments each. For each experiment, CerS isoform-specific mRNA down-regulation was confirmed by real-time qPCR.