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. Author manuscript; available in PMC: 2015 Mar 4.
Published in final edited form as: Cell Signal. 2010 Apr 18;22(9):1300–1307. doi: 10.1016/j.cellsig.2010.04.006

Fig. 5. CerS5 and 6 are both required for ionizing radiation-induced palmitoyl-CoA-dependent CerS activity.

Fig. 5

(A) Proteins from HeLa cell subcellular fractions were analyzed by quantitative IP western blotting for presence of CerS6, as detailed in Materials and Methods. (B) Kinetic analysis of palmitoyl-CoA-dependent CerS activity measured in homogenates prepared from unirradiated and irradiated (20 Gy) HeLa cells transfected with shRNA to either CerS5 or CerS6, or both. (C) Kinetics of palmitoyl-CoA-dependent CerS activity measured in MAM-enriched fractions purified from unirradiated and irradiated (10 Gy) HeLa cells transfected with shRNA to either CerS5 or CerS6. Data represent 1 of at least 2 similar experiments. For each experiment, CerS gene-specific mRNA down-regulation was confirmed by real-time qPCR.