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. Author manuscript; available in PMC: 2015 Mar 4.
Published in final edited form as: Mol Microbiol. 2011 Nov 7;82(5):1204–1216. doi: 10.1111/j.1365-2958.2011.07879.x

Fig. 2.

Fig. 2

49E10 develops into bradyzoites and the disrupted mRNA is not downregulated

A. Immunofluorescent images of E10 and 49E10 parasites grown in bradyzoite inducing conditions for three days. Cells were fixed and stained with DBA to mark the cyst wall (green) and BAG1 (red). All coverslips were mounted with VectaShield mounting medium containing 4′6′-diamidino-2-phenylindole (DAPI, Vector Laboratories) to visualize DNA (blue). Fluorescent images were acquired as described (Mordue et al., 2007).

B. Total RNA from E10 and 49E10 parasites was analyzed by northern blot analysis using a probe upstream of the insertion site of TGME49_023970 in 49E10 mutant (top panel labeled EFG). The blot was striped and reprobed for TUB1 as a loading control (bottom panel labeled TUB). Numbers in the left column are the sizes of the markers in kilobases.