Fig. 5.

Insertion into the 3′UTR of TgEFG affects protein levels

A. 1×10⁷ tachyzoites of clones expressing TgEFG-HA with either an endogenous 3′UTR (WT 3′UTR) or Mutant 3′UTR were loaded onto a 10% SDS/PAGE gel. Protein was visualized using a mouse anti-HA monoclonal antibody to detect the HA epitope tagged TgEFG (top panel labeled TgEFG). The blot was striped and reprobed for ß-tubulin as a loading control (bottom panel labeled ß-TUB). Size markers are shown to the left in kilodaltons.

B. The virulence of E10 (circle) and 49E10 (diamond) parasites was compared to two independent clones of 49E10 complemented with TgEFG expressed with its endogeneous 3′UTR (WT 3′UTR), or the mutant 3′UTR found in 49E10 (Mut 3′UTR). For this acute mouse model, 1.5×10⁶ tachyzoites were i.p. injected into four mice per strain. Two independent experiments were combined for a total of eight mice per strain.