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. Author manuscript; available in PMC: 2015 Mar 4.
Published in final edited form as: Mol Microbiol. 2011 Nov 7;82(5):1204–1216. doi: 10.1111/j.1365-2958.2011.07879.x

Fig. 6.

Fig. 6

TgEFG mRNA levels in 49E10 are only altered in vivo

A. Quantitative RT-PCR was performed on RNA from in vitro grown E10 and 49E10 parasites using primers against TgEFG and α-tubulin. Standard curves were generated using the same primers to determine the copy number of TgEFG and α-tubulin in each sample and the ratio of the TgEFG copy number to α-tubulin copy number was determined. The average of three independent experiments is shown.

B. Quantitative RT-PCR was performed on RNA from in vivo derived E10 and 49E10 parasites as in A. (* p=0.0042).