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. Author manuscript; available in PMC: 2015 Mar 4.
Published in final edited form as: Adv Cancer Res. 2012;115:1–38. doi: 10.1016/B978-0-12-398342-8.00001-X

Figure 1.2.

Figure 1.2

Constructing a CTV using recombinant DNA techniques. The cancer-specific PEG-Prom-driven E1A gene (PEG-Prom-E1A) and the CMV promoter-driven mda-7/IL-24 or IFN-γ gene (CMV-mda-7 or CMV-IFN-γ) were cloned into the multiple cloning site of the shuttle vectors pE1.2 and pE3.1, respectively. The cassettes containing the promoter and the transgene were further digested by different restriction enzymes as shown in the figure and ligated into SfiI-digested adenoviral vector. Taken from Sarkar, Su, Vozhilla, Park, Randolph, et al. (2005).