FIG. 8.

Detection of T-tau and P-tau in human serum post severe traumatic brain injury (sTBI) by enhanced immunoassay using multi-arrayed fiberoptics coupled to rolling circle amplification (a-EIMAF). Human serum samples were diluted 1:20 in phosphate-buffered saline and assayed by a-EIMAF for T-tau (Mab DA31) and P-tau (Mab RZ3) in combination with biotinylated Mab DA9 for detection. Serum levels of both T-tau and P-tau are elevated in the acute phase (range, Days 1–3), compared with control serum. In the chronic serum samples (range, Days 27–190), the majority of T-tau returned to control levels. In contrast, P-tau levels in most sTBI serum samples remained higher than control levels.