Figure 8. The local proliferation of endogenous Ag-specific CD8+ T cells in the vaginal mucosa of Ivag immunized WT mice with or without FTY720 treatment.
The BrdU+ Ag-specific CD8+ T cells in vaginal mucosa of FTY720 treated or untreated immunized mice. ○, naïve mice (n=3); ●, BrdU-treated naïve mice (n=3); □, immunized mice (n=5); ■, BrdU-treated immunized mice (n=5); △, FTY720 and BrdU-treated immunized mice (n=5). This is representative of 2 independently repeated experiments with the same results. (a) The experimental design. (b) The absolute number (mean ± SEM) of BrdU+ Ag-specific CD8+ T cells in vaginal mucosa and iliac LNs. The p values were generated using a one-way ANOVA plus Tukey’s multiple comparison.