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. 2015 Feb 22;15:43. doi: 10.1186/s12866-015-0373-0

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© Tanaka et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Northern blotting analysis of rocG transcripts in the presence and absence of lctE. RNAs were prepared from 168 (lane 1), TM023 (ccpA::neo, lane 2), MY02 (lctE::spc, lane 3), 1A95 (degU32, lane 4), TM024 (degU32 ccpA::neo, lane 5), and KI004 (degU32 lctE::spc, lane 6) cells for northern blotting analyses using probes for rocG transcripts under high-stringency conditions. Experiments were repeated independently at least three times with similar results, and representative data are shown. At the bottom, rRNAs (23S and 16S) were visualized as loading controls using methylene blue staining.