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. 2015 Jan 24;12:14. doi: 10.1186/s12974-014-0223-2

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© Hernandez-Encinas et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Effect of lipopolysaccharide (LPS) on the expression of C/EBPβ and C3 in the SNpc of the rat. Rats were injected with LPS in the SNpc and 72 hours later the expression of C/EBPβ and C3 was determined as indicated in Methods. (A) Immunohistochemistry analysis of coronal brain sections showing C/EBPβ and C3 expression in the SNpc of adult rats injected or not with LPS. Labeling was performed using specific antibodies recognizing C/EBPβ (red) and C3 (green). Nuclei were stained with DAPI (blue). (B) Immunohistochemistry analysis of coronal brain sections showing C/EBPβ and TH expression in astrocytes and microglia cells in the SNpc of adult rats injected or not with LPS. Labeling was performed with tomato lectin (to identify microglial cells) and specific antibodies recognizing GFAP (astrocytes, blue), C/EBPβ (green) and TH (magenta). (C) Quantification of the number of double stained C/EBPβ/tomato-lectin⁺ and C/EBPβ/GFAP⁺ cells in the SNpc. Values are expressed as a percentage of double C/EBPβ/tomato-lectin⁺ and C/EBPβ/GFAP⁺ inmmunoreactive cells with respect to the total number of microglial or astroglial cells, respectively.