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. Author manuscript; available in PMC: 2016 Mar 2.
Published in final edited form as: Curr Biol. 2015 Feb 5;25(5):671–677. doi: 10.1016/j.cub.2015.01.059

Figure 1. KMN network components display separable recruitment to CENP-T.

Figure 1

A) Immunofluorescence images showing positive (GFP-CENP-C (1-100)-LacI) or negative (GFP-CENP-C (101-234)-LacI) co-localization with anti-Ndc80 in nocodazole-treated cells. Chosen cells lacked overlap between the GFP focus and endogenous kinetochores marked by anti-CENP-A. Images were scaled independently to show the full range of data. Arrowheads indicate position of the GFP focus. B) Summary of immunofluorescence experiments assessing co-localization of KMN components with the indicated CENP-C-LacI fusions. >90% of cells display indicated behavior (N = 50 cells/condition). C) Representative immunofluorescence images showing localization of GFP-CENP-T-LacI foci and KMN components in nocodazole-arrested mitotic cells. Images were scaled independently to show full range of data. Numbers in lower right indicate number of mitotic cells showing co-localization. D) Summary of co-localization of KMN components with CENP-T-LacI fusions. >90% of cells observed display indicated behavior (N = 50 cells/condition). Scale bars, 5 μm. See also Figures S1 and S2.