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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: Clin Cancer Res. 2014 Dec 23;21(5):1010–1018. doi: 10.1158/1078-0432.CCR-14-1956

Figure 1. Autologous, GM-CSF secreting leukemia cell vaccines early after allogeneic HSCT stimulate antibodies to angiogenic cytokines.

Figure 1

Representative examples are shown for longitudinal studies of responses to L1CAM, Del-1, angiopoietin-1, angiopoietin-2, VEGF-A, progranulin, PDGF-BB, and hepatocyte growth factor. Sera samples obtained after allogeneic HSCT were diluted 1:2000 and tested for reactivity against recombinant His-tagged angiogenic proteins or a 6xHis control. A horseradish peroxidase conjugated anti-human IgG (Fab)2 was used to detect the patient antibodies, and Tyramide was added to amplify the signal. Absorbance was measured at 450 nm. Arrows along the abscissa denote individual vaccinations. Similar results were observed in at least three independent assays.