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. 2015 Mar 4;35(9):3893–3902. doi: 10.1523/JNEUROSCI.4402-14.2015

Figure 5.

Figure 5.

Coronin-1 and PLC-γ1 work in concert to support calcium-dependent inhibition of axon growth and branching. A, PLC-γ1 inhibitor has a similar effect as BAPTA-AM on NGF-induced axon growth. Axon growth assays were performed on neurons from WT or Coro1a−/− mice grown in NGF (2 ng/ml) with or without U73122 (1 μm) as described in Figure 1B. WT control (n = 28), WT with U73122 and NGF (n = 31), Coro1a−/− control (n = 29), and Coro1a−/− with LY294002 and NGF (n = 35). B, D, PLC-γ1 inhibitor has a similar effect as BAPTA-AM on NGF-induced axon branching. Branching of WT or Coro1a−/− mice grown in NGF (2 ng/ml) with or without U73122 (1 μm) as described in Figure 1F. Scale bar, 150 μm. C, E, Sholl analysis quantification of B and D (n = 7). Error bars represent SEM; *p < 0.05 using unpaired two-tailed Student's t test.