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. 2015 Mar 3;10(3):e0118414. doi: 10.1371/journal.pone.0118414

Fig 3. Ligand binding by rRs-FAR-1.

Fig 3

(A) Fluorescence emission spectra (excitation at 345 nm) of DAUDA either alone or after the addition of rRs-FAR-1. A reverse change in DAUDA emission was observed after the addition of oleic acid to the rRs-FAR-1+DAUDA complex. The wavelengths of peak emission by DAUDA were different. (B) Fluorescence emission spectra (excitation at 350 nm) of retinol in ethanol either alone or after the addition of rRs-FAR-1. A competitive effect of oleic acid was also observed. (C) Fluorescence emission spectra (excitation at 319 nm) of cis-parinaric acid either alone or after the addition of rRs-FAR-1. The effects of the subsequent addition of retinol to rRs-FAR-1+cis-parinaric acid and the addition of an oleic acid solution to rRs-FAR-1+cis-parinaric acid+retinol were examined.