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. 2015 Jan 7;6(3):229–233. doi: 10.1007/s13238-014-0129-x

Figure 1.

Figure 1

F2Y was incorporated at Tyr574 site of ETK-FL protein. (A) Topology diagram of an ETK protein in lipid bilayer, containing two transmembrane helices and a cytosolic tyrosine kinase domain including the auto-phosphorylation site Y574 and tyrosine rich C-terminal tail; (B) Coomassie blue staining of purified ETK-Y574F2Y; (C) Western-blot of purified ETK-Y574F2Y