Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Defective loading of Pri2 and Pol α onto early replication origins in the pri2 ( C434A ) mutant. Congenic wild-type (POL1-Myc, PRI2-HA) and mutant (POL1-Myc, pri2(C434A)-HA) cells were synchronized in G1 with α factor and released at 23°C. Cells were collected at the indicated time points for FACS (A) and ChIP (B) analyses as described in Fig. 3. Pol1- and Pri2-associated chromatin was immunoprecipitated using anti-Myc and anti-HA monoclonal antibodies, respectively
