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. Author manuscript; available in PMC: 2016 Mar 3.
Published in final edited form as: Biochemistry. 2015 Feb 17;54(8):1673–1680. doi: 10.1021/bi501420n

Figure 3.

Figure 3

Enzyme activity of CKB (■) and CKB+ASB9(1-252) (●) at 3 nM enzyme concentration and 30° C. The KM was 2.1 ± 0.2 mM and the Vmax was 3.36 ± 0.07 × 10−7 Ms−1 (for comparison to literature values this translated to kcat = 6.7×103 min−1). ASB9(1-252) binding to CKB partially inhibits the activity of CKB increasing the KM slightly to 3.4 ± 0.3 mM and decreasing the Vmax to 2.1 ± 0.06 × 10−7 Ms−1.