Table 1. Quantitative parameters for Texas Red-labeled lysine-fixable dextran tracers.
| TR-Dex3 | TR-Dex10 | |
|---|---|---|
| Approximate molecular weight (Da) | 3,000 | 10,000 |
| Free diffusion coefficient, D ( × 10−7 cm2/second; 37±0.5 °C) | 24.6±0.20 (12) | 15.6±0.15 (13) |
| Hydrodynamic diameter, dH (nm)a | 2.67±0.02 | 4.21±0.04 |
| Endpoint plasma sampling time after administration start (minutes) | 20 | 30 |
| Intranasal dextran concentration (mg/mL)b | 25 | 50 |
| Intranasal dextran dosing | 4 × 12 μL drops | 4 × 6 μL drops |
| Endpoint [plasma]intranasal (ng/mL) | 21.1±1.8 (5) | 11.5±1.4 (5)c 13.1±1.8 (6)d |
| AUCintranasal (nmol/L·minutes) | 48.7±13.1 (5) | 16.1±2.3 (5)c 17.9±3.7 (6)d |
| Intraarterial dextran concentration (μg/mL)b | 4 | 0.4 |
| Intraarterial dextran dosing | 500 μL bolus | 500 μL bolus |
| Endpoint [plasma]intraarterial (ng/mL) | 92.8±13.7 (5) | 7.5±0.3 (3)e |
| AUCintraarterial (nmol/L·minutes) | 934.6±89.2 (5) | 36.7±0.9 (3)e |
Values reported as mean±s.e.m. (n independent measurements).
AUC, area under the plasma concentration-time curve from the start of dextran administration until the endpoint sample collection; TR-Dex3, Texas Red-labeled 3 kDa dextran.
a
Apparent hydrodynamic diameter determined from Stokes–Einstein equation [dH=(kT)/(3πηD), where k is Boltzmann's constant, T is absolute temperature, and η is the viscosity of water (6.9152 × 10−4 Pa second at T=310 K)].26
b
Dissolved in saline.
c
Intranasal saline (4 × 6 μL drops) followed by intranasal TR-Dex10.
d
Intranasal matrix metalloproteinase-9 (100 nmol/L; 4 × 6 μL drops) followed by intranasal TR-Dex10.
e
Intranasal matrix metalloproteinase-9 (100 nmol/L; 4 × 6 μL drops) followed by intraarterial TR-Dex10.