Fig. 1.

P2X4R and eNOS physically associate with each other as evidenced by co-immunoprecipitation and co-localization in cardiac ventricular myocytes of WT and P2X4R Tg mice. (a) WT myocyte lysates were incubated with anti-eNOS antibody or with non-specific IgG as control. The isolated complex was probed with eNOS (top panel) and P2X4 (bottom panel) antibodies using western blotting. In a control experiment, eNOS co-immunoprecipitated itself. Co-immunoprecipitation of P2X4R with eNOS antibody (lane 3) but not with control IgG (lane 2) was shown. (b) Same experiment as in (a) conducted in P2X4-Tg myocytes. (c) Immunostaining of eNOS (green), P2X4R (red), and merged image was shown for a P2X4R-overexpressing Tg cardiac myocyte.

The figure and its legend are reproduced with permission from Wolters Kluwer Health (see  Acknowledgment).