Figure 3.
Lymphocyte-activation gene 3 (LAG3) transcription levels in Mycobacterium tuberculosis (MTB)–infected nonhuman primates undergoing activation of tuberculosis (TB). The fold change of LAG3 mRNA expression was measured using quantitative RT-PCR through the calculation of the 2−ΔΔCT, where normalization was calculated with β-actin and corresponding uninfected tissue. LAG3 transcription levels were compared between lung tissue of active (n = 10), reactivated (n = 7), and latent tuberculosis infection (n = 8) MTB infections (A); active MTB (n = 10) and Brucella infections (n = 6) (B); and reactivated MTB infections (n = 7) and simian immunodeficiency virus (SIV) infections (n = 4) (C); BAL of active (n = 4), reactivated (n = 7), and LTBI (n = 4) MTB infections (D); spleen of active (n = 4), reactivated (n = 5), and LTBI (n = 5) MTB infections (E); and bronchial lymph node (BrLN) of active (n = 4), reactivated (n = 4), and LTBI (n = 4) MTB infections (F). Data points are categorized by infection type, where circles indicate active TB; squares, reactivated TB; triangles, LTBI; clear circles, SIV alone; and upside-down triangles, Brucella infections. Each point represents one data point, and the means ± SD are represented by horizontal bars. Statistical significance was determined by either a one-way analysis of variance (A and D–F) or a two-tailed Student's t-test (B and C) in GraphPad Prism 6. ∗P < 0.05.