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. 2015 Mar;185(3):820–833. doi: 10.1016/j.ajpath.2014.11.003

Figure 6.

Figure 6

Identification of cellular subsets expressing lymphocyte-activation gene 3 (LAG3) in the lung granulomas of macaques with active tuberculosis. Optimal cutting temperature–frozen and stimulated lung tissue sections were assessed using immunofluorescence staining and confocal microscopy imaging. A and B: Images were stained for T cells with LAG3 (green), CD3 (red), and TO-PRO (blue) nuclear stain at low and high magnification. C: Natural killer cells were stained using a negative selection strategy with LAG3 (green), granzyme B (red), and CD3 (blue). D: Alveolar macrophages do not express LAG3 [using markers for LAG3 (green), Ham56 (red), and TO-PRO (blue)].