Figure 3.

p90 ribosomal S6 kinase (RSK) inhibition decreases nodular melanoma (NM) cell migration and invasion. A: WM 278 and WM 3248 cells treated with equal amounts of dimethyl sulfoxide (DMSO) or 5 μmol/L BI-D1870 migrated through 8-μm migration chambers for 6 hours and then were fixed and stained with crystal violet. Five high-power fields from each chamber were photographed, and cells were then counted. Images shown are representative of one high-power field from each condition. B: Same as A but chamber coated with Matrigel and cells allowed to invade for 20 hours. For A and B, data were normalized by setting DMSO-treated cells to 100%. C: Same as B but using siRNA against RSK1. Data are given as means ± SEM. ^∗∗∗P < 0.001 compared with DMSO-treated cells (A and B) or with scrambled siRNA–treated cells (C).