Skip to main content
. 2015 Mar;185(3):704–716. doi: 10.1016/j.ajpath.2014.11.021

Figure 5.

Figure 5

Constitutive activation of protein S6 kinase, 90 kDa, polypeptide 1 (RSK1) accelerates melanoma tumor onset and invasion in zebrafish. A: Melanoma-free survival curve of miniCoopR-green fluorescent protein (GFP)–, miniCoopR-Myr-RSK1–, and miniCoopR-RSK-deltaCCT–injected zebrafish. B: Histologic sections of zebrafish RSK1dCCT- and Myr-RSK1–expressing melanomas were stained with hematoxylin and eosin to assess tumor invasion depth. Arrows indicate muscular invasion by melanoma cells. C: Depth of invasion was measured for each tumor and was quantified by averaging the depth of invasion of four tumors for each group (RSK1dCCT and Myr-RSK1). Data are given as means ± SEM. ∗∗∗P < 0.001 compared with dominant-negative RSK1-expressing tumors.