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. 2015 Mar;185(3):741–755. doi: 10.1016/j.ajpath.2014.11.006

Figure 3.

Figure 3

Cigarette smoke (CS) exposure increases lymphoid aggregate counts in nonhuman primate (NHP) lungs. A: Hematoxylin and eosin staining of lung sections from NHPs exposed to air or CS for 12 weeks. Top, middle, and bottom panels: Lymphoid aggregates in or around lung parenchyma, vessels, and bronchi, respectively, are indicated by arrows. B–D: Quantitation of lymphoid aggregates in parenchyma (B), and around vessels (C) and bronchi (D) in the lungs of air- and CS-exposed NHPs normalized to the area of the tissue in each microscopic field (30 randomly captured fields per animal). E: Triple-color immunofluorescence staining of a lymphoid aggregate in a representative lung section from a CS-exposed NHP. B lymphocytes are identified with a green fluorophore; CD8+ T lymphocytes, a red fluorophore; and CD4+ T lymphocytes, a yellow fluorophore. Data are presented as means ± SEM (B–D). n = 7 NHPs per group (B–D). ∗P < 0.05 versus the air-exposed NHPs (B–D). Original magnification, ×400 (A and E).