Skip to main content
. 2015 Mar;185(3):741–755. doi: 10.1016/j.ajpath.2014.11.006

Figure 7.

Figure 7

Cigarette smoke (CS) exposure induces alveolar septal cell apoptosis in peripheral lung. Nonhuman primates (NHPs) were exposed to air or CS for 12 weeks, and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining was performed on formalin-fixed lung sections. A: Quantification of TUNEL-positive cells in air- and CS-exposed groups normalized to unit area of the alveolar wall. B, top panels: Representative images of TUNEL staining of lung sections from air- and CS-exposed NHPs. Arrows indicate TUNEL-positive cells. B, bottom panel: Lung sections from CS-exposed NHPs that were subjected to TUNEL staining with the terminal deoxynucleotidyl transferase (TdT) enzyme omitted as a negative control. C: Quantification of active caspase-3–positive alveolar septal cells in air- and CS-exposed NHP lungs normalized to area of lung tissue. D, top panels: Representative images of active caspase-3 staining in the lungs of air- and CS-exposed NHPs. Arrows indicate active caspase-3–positive cells. D, bottom panel: Lung sections from CS-exposed NHPs immunostained with isotype-matched murine (Ms) control primary IgG antibody. Data are presented as means ± SEM (A and C). n = 4 air-exposed NHPs and n = 5 CS-exposed NHPs per group (A); n = 5 NHPs per group (C). ∗P < 0.05 versus air-exposed NHPs (A and C). Original magnification, ×600 (D).