Fig. 4.

C7 is required for C5b-9 formation. Serum-deprived and anti-CD59 treated 661W cells were treated with either C7-depleted NHS (10 or 20 % for 60 min) or C7-depleted serum supplemented with purified exogenous C7 (100 µg/ml) (10 or 20 % for 60 min). Immunofluorescence staining and confocal imaging of C5b-9 and F-actin shows that in cells treated with C7 deficient serum, cellular morphology was normal with abundant F-actin stress fibres, and no evidence of C5b-9 staining. Restoration of C7 led to the appearance of punctate C5b-9 staining, and characteristic disruption of the F-actin cytoskeleton. DAPI was used to stain the nuclei. Scale bar 10 μm. All experiments were repeated at least three times, and representative images are shown