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. 2015 Jan 8;8(3):253–269. doi: 10.1242/dmm.017756

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© 2015. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — Inducibility of a subset of cxcl11-like genes upon bacterial infection. (A,B) Quadruplicate pools of 18–20 embryos infected in the hindbrain with 200 CFU of M. marinum (Mm) or S. typhimurium (St) at 30 hpf or mock-injected with PBS were collected at 1 hpi (A) and 3 hpi (B). A subset of CXCL11-like chemokine genes shows upregulation by qPCR in the infected groups, which becomes significant at 3 hpi. Error bars: mean±s.e.m. (C,D) Triplicate pools of 18–20 embryos systemically infected with M. marinum (Mm) at 1 dpf or mock-injected with PBS were collected at 4 hpi (C) and 4 dpi (D). Expression of cxcl11aa and cxcl11ae was significantly induced at both time points, whereas cxcl11ac, cxcl11ad and cxcl11af/ag were significantly induced only at 4 dpi. Error bars: mean±s.e.m. *P<0.05; **P<0.01.