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. 2015 Jan 29;8(3):295–309. doi: 10.1242/dmm.018754

Fig. 4.

Fig. 4.

apoEa localization to the YSL and intestine contrasts with widespread expression of apoEb. apoEa and apoEb mRNA expression observed by ISH during blastulation [30% epiboly (E)], gastrulation (80% E), somitogenesis [15–20 somite (S)] and 1–6 dpf. apoEa mRNA is expressed in distinct subregions of the YSL at somitogenesis and 1 dpf, and ubiquitously throughout the YSL from 2–5 dpf. At 6 dpf (but not 5 dpf), apoEa is observed in the intestine (I). apoEb is expressed not only throughout the YSL (30% E to 5 dpf), but also in the tail bud (15–20 S and 1 dpf), the apical ectodermal ridge (AER) (1–6 dpf), the head (H) (1–6 dpf), mouth and nose orifices (O) (2–6 dpf), the find buds (FB) (2–6 dpf), pharynx (P) (2–6 dpf), gill arches (GA) (3–6 dpf), periderm (4–6 dpf), retina (R) (4–6 dpf), intestine (6 dpf) and swim bladder (6 dpf). No expression of apoEa was observed during blastulation or gastrulation, or for either gene at the eight-cell stage (supplementary material Fig. S3). Zebrafish are wild type, except for 6-dpf larvae which are nacre−/−; 1–6 dpf larvae were treated with hydrogen peroxide to remove pigmentation. ISH was performed in triplicate with n≥5 embryos or larvae per probe per experiment.