Fig. 7.

Overexpression of zebrafish ApoA-IVb.1 decreases food intake. (A) Representative fluorescent images of spotted lipid extracts; each spot represents the fluorescent lipids of 10 pooled larvae following lipid extraction. Tg represents Tg(hsp70:apoA-IVb.1:mCherry) larvae and WT represents wild-type larvae. Larvae have either never eaten exogenous food (Unfed), or have been fed a high-fat, 10% chicken egg yolk with 6.4 μM BODIPY® FL C₁₆ for 4 hours (Fed). (B) Tg(hsp70:apoA-IVb.1:mCherry) larvae ingest fewer fluorescently labeled lipids as shown by comparison of arbitrary fluorescent units (a.f.u.) to wild type (paired Student’s t-test, P<0.001; n=9, 20 larvae per experiment). Total larval lipids were extracted and lipid fluorescence was measured. Natural fluorescent lipid background was corrected using measurements of unfed larvae. The box represents the 25–75th percentiles, and the median is indicated. The whiskers show the 10–90th percentiles.