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. 2015 Mar 4;5:8759. doi: 10.1038/srep08759

Figure 1.

Figure 1

(a) Peptide group-specific antibodies. TXP antibodies target peptide subsets of global tryptic digests that share the same terminus. (b) Frequency of C-termini in a peptide pool immunoprecipitated by the antibody anti-LPNK. Peptides were immunoprecipitated from 50 μg tryptically digested hepatocyte and analyzed by high resolution tandem-mass spectrometry. Theoretical number of peptides harboring the respective C-termini were calculated from an in silico digest of the Uniprot database (UniProt Homo Sapiens Reference Proteome Set database (2014 Release 1)). (c) Amino acid frequency in the binding motif of the antibody, calculated based on the high resolution tandem-mass spectrometry analysis.