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. 2015 Mar 4;5:8733. doi: 10.1038/srep08733

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(A–D). Cells isolated from BAL were stained with anti-CD68 and anti-IL-10 antibodies, and an aliquot of 10,000 cells was analyzed using flow cytometry. Cells were initially gated on forward and side scatter to remove debris and calculated using quadrant dot plots. (F). The percentage of CD68-positive cells (lower right and upper right window) in each group was plotted. The proportion of CD68-positive macrophages was significantly elevated by approximately 30% following CS treatment compared with the Sham group, and the CD68-positive cell population was reduced in the presence of MSCs. Within the CD68-positive population, the percentage of IL-10-positive (CD68⁺IL-10⁺) cells inceased by approximately 13% in the CS + MSCs group compared with the CS group. Data represent the mean ± SEM of 5 rats in each group from a single experiment and are representative of two independent experiments (n = 5) with similar results. *significant difference (P < 0.05) between the CS and CS + MSCs groups.