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. 2015 Mar 4;5:8733. doi: 10.1038/srep08733

Figure 5. MSCs attenuated CS-induced COX-2, PGE2, IL-6, and iNOS production and promoted IL-10 production in macrophages.

Figure 5

(A–D). Macrophages were cultured with or without MSCs in 10% CSE for 48 h and were subjected to real-time PCR analysis. CSE significantly increased the COX-2, IL-6 and iNOS mRNA expression and decreased the IL-10 mRNA expression over the vehicle control. In the MSC treatment group, the mRNA levels of COX-2, IL-6 and iNOS were decreased significantly, while the IL-10 mRNA levels increased slightly. (E–G). The culture supernatants were retained and subjected to ELISA. The PGE2 and IL-6 levels increased in supernatants and decreased after MSC treatment. However, IL-10 protein decreased in supernatants and increased after MSC treatment. Data represent the mean ± SEM of n = 5/group, *significant difference (P < 0.05), **significant difference (P < 0.01), between CS and CS + MSCs group.