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. 2015 Mar 4;5:8733. doi: 10.1038/srep08733

Figure 6. MSC treatment inhibited CS-induced phosphorylation of p38 MAPK and ERK, but not JNK in macrophages.

Figure 6

Macrophages were treated with 10% CSE for 0–40 min, and MAPK phosphorylation was assessed in cytoplasmic extracts by western blot analysis. (A). Macrophages were probed for activation-associated phosphorylated p38 MAPK and total p38 MAPK. p38 MAPK was phosphorylated after 20 min with maximal phosphorylation at 30 min in response to CSE stimulation; the phosphorylation was inhibited by MSC treatment. (B). ERK was phosphorylated after 20 min with highest phosphorylation at 30 min in response to CSE stimulation; the phosphorylation could be inhibited by MSC treatment. (C). JNK was phosphorylated from 20 min with highest phosphorylation at 30 min in response to CSE. However, MSCs could not inhibit the activation-associated phosphorylation of JNK. Blots were representative of at least four separate experiments. Data represent the mean ± SEM, #significant difference (P < 0.05), ##significant difference (P < 0.01), versus corresponding untreated controls; *significant difference (P < 0.05), **significant difference (P < 0.01), versus corresponding MSCs(−) counterparts. (Full-length blots were presented in Supplementary Fig S6.)