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. 2015 Mar 4;5:8755. doi: 10.1038/srep08755

Figure 3. βA3/A1-crystallin modulates the phosphorylation of STAT3 in astrocytes.

Figure 3

(a). Immunoblot analysis of total and p-STAT3 in WT astrocytes upon treatment with IL-6 and Stattic showed a 2.1 fold increase in the levels of p-STAT3 in astrocytes treated with IL-6 and ~70% reduction in p-STAT3 upon treatment with Stattic compared to vehicle-treated astrocytes. A ~70% reduction in p-STAT3 in astrocytes pre-treated with IL-6 upon treatment with Stattic compared to astrocytes treated with IL-6 alone was observed. (b). Analysis of p-STAT3 levels by ELISA revealed 5 fold induction of p-STAT3 in the presence of IL-6 and ~76% reduction in the presence of Stattic compared to the vehicle control. Stattic significantly decreased the levels of p-STAT3 in the presence of IL-6 (~85%) compared to the astrocytes treated with IL-6 alone. (c). However, in Cryba1 KD astrocytes there was only modest activation (1.3 fold) of p-STAT3 in the presence of IL-6. Significant inhibition of p-STAT3 was observed in the presence of Stattic. (d). ELISA data revealed that IL-6 treatment induced p-STAT3 by 4.5 fold in Cryba1 KD astrocytes compared to vehicle-treated astrocytes. Stattic decreased the level of p-STAT3 by ~80% when treated alone and by ~85% when treated in combination with IL-6 compared to vehicle-treated and IL-6 treated Cryba1 KD astrocytes. Error bars indicate s.d.; *P<0.05.