Skip to main content
. 2015 Mar 4;5:8735. doi: 10.1038/srep08735

Figure 1. miR-320a directly targets ARPP-19 and ERRγ in MCF-7 cell line.

Figure 1

(a). miR-320a expression levels were examined in 58 fresh tissues including 31 breast cancer and 27 normal breast samples from patients using real-time PCR. (b). Putative-binding sites for human (hsa) miR-320a were predicted in the 3′UTR of ARPP-19 and ERRγ mRNA and mutation sites. (c). Real-time PCR analysis of miR-320a expression in MCF-7 cells when transfected with miR-320a mimics or inhibitors. (d). Luciferase activity of luciferase gene fusing with wild-type or mutant 3′UTRs of ARPP-19 or ERRγ were measured after co-transfection with miR-320a mimics or inhibitors in MCF-7 cell line. The luciferase activity was normalized with firefly luciferase activity. (e). Western blot analysis of the expression levels of ARPP-19 and ERRγ in MCF-7 cells transfected with miR-320a mimics or inhibitors and the densitometric analysis of ERRγ protein levels. Results are presented as an average of at least three replicates.*P < 0.05, **P < 0.01, ***P < 0.001.