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. 2015 Mar 4;5:8735. doi: 10.1038/srep08735

Figure 4. Re-expression of miR-320a or knock-down of its targets in TamR cells sensitizes these cells to tamoxifen.

Figure 4

(a) and (b). Cell viability (a) and soft agar colony formation (b) analysis of TamR cells transfected with miR-320a mimics. (c). The expression of ARPP-19, ERRγ, c-Myc, and Cyclin D1 was examined by Western blot after transfected with indicated siRNA or siRNA and plasmids for 48 h. ARPP-19, c-Myc, and Cyclin D1 were labeled with FLAG tag and ERRγ was labeled with GFP tag. d and e. Cell viability (d) and soft agar colony formation (e) analysis of TamR cells transfected with si-ARPP-19, -ERRγ, -c-Myc, and -Cyclin D1. Results are shown as the average and s.d. of at least three biological and two technical replicates each,*P < 0.05, **P < 0.01, ***P < 0.001.