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. 2015 Mar 4;5:8735. doi: 10.1038/srep08735

Figure 6. c-Myc mediates progesterone (P4) and estrogen (E2)-regulated miR-320a expression.

Figure 6

(a). Real-time PCR analysis of miR-320a expression levels in MCF-7 cells treated with E2 or P4 for 0 h, 12 h, and 24 h. (b) and (c). Immunoblot analysis (the left panel) of ARPP-19, ERRγ, c-Myc, and Cyclin D1 levels in MCF-7 cells transfected with miR-320a mimics/inhibitors in the presence or absence of E2 (b) or P4 (c) treatment and the corresponding densitometric analysis (the right panel). (d). Real-time PCR analysis of miR-320a expression levels in MCF-7 cells transfected with either c-Myc specific siRNA or c-Myc-flag (c-Myc) overexpression plasmids. e and f. Real-time PCR analysis of miR-320a expression levels in MCF-7 cells transfected with si-c-Myc (e)/c-Myc overexpression plasmids (f) for 24 h and followed with stimulation with E2 (e)/P4 (f). Results are presented as an average of at least three replicates. *P < 0.05, **P < 0.01, ***P < 0.001.